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- The A.thaliana Co-Response Database -		 AthCoR@CSB.DB
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NOTIFICATION: Migration to a new permanent home at wwwcsbdb.de.
Herewith we inform you that all CSB.DB databases have been migrated at the beginning of 2016. This includes all gene correlation and expression databases (search the WWW for alternatives), the GMD@CSB.DB module (alternative will be reachable via http://gmd.mpimp-golm.mpg.de/) and all associated databases (terminated).
The BestFit software, a tool for non-aqueous fractionation data analysis, will be available by the Experimental Systems Biology Research Group headed by Dr. Patrick Giavalisco.
We thank all users, contributors, and collaborators of CSB.DB at the Max Planck Institute for their long-standing support.
Yours sincerely, the CSB.DB Curator and the CSB.DB Developmental Core Team
    
down EXPERIMENT
down SAMPLE(S)
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11700
 

Replicate Set Accession: rs_r11710
Experiment Accession11700
Replicate Set Accessionrs_r11710
Biosample Accessionbs_r11710
Biomaterial Descriptionseedling, roots
Biomaterial Is Control?False
Growth DescriptionSeeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C).
Treatment DescriptionDrought stress: The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels back in the climate chamber. Harvested 12 h after start of experiment
Anatomy Descriptionall roots
Development Description16 days old plants, roots
Probe Typetotal RNA
Germplasm NameCol-0
Germplasm TAIR Accession1092
Replicate Set Typebiological
Is Multichannel?False
Is Recerse Dye?False
Scanning SoftwareGCOS
Array DesignATH1-121501
Number Of Slides2


Replicate Set Accession: rs_r11706
Experiment Accession11700
Replicate Set Accessionrs_r11706
Biosample Accessionbs_r11706
Biomaterial Descriptionseedling, roots
Biomaterial Is Control?False
Growth DescriptionSeeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C).
Treatment DescriptionDrought stress: The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels back in the climate chamber. Harvested 3 h after start of experiment
Anatomy Descriptionall roots
Development Description16 days old plants, roots
Probe Typetotal RNA
Germplasm NameCol-0
Germplasm TAIR Accession1092
Replicate Set Typebiological
Is Multichannel?False
Is Recerse Dye?False
Scanning SoftwareGCOS
Array DesignATH1-121501
Number Of Slides2


Replicate Set Accession: rs_r11711
Experiment Accession11700
Replicate Set Accessionrs_r11711
Biosample Accessionbs_r11711
Biomaterial Descriptionseedling, green parts
Biomaterial Is Control?False
Growth DescriptionSeeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C).
Treatment DescriptionDrought stress: The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels back in the climate chamber. Harvested 24 h after start of experiment
Anatomy Descriptionall green parts
Development Description16 days old plants, green parts
Probe Typetotal RNA
Germplasm NameCol-0
Germplasm TAIR Accession1092
Replicate Set Typebiological
Is Multichannel?False
Is Recerse Dye?False
Scanning SoftwareGCOS
Array DesignATH1-121501
Number Of Slides2


Replicate Set Accession: rs_r11705
Experiment Accession11700
Replicate Set Accessionrs_r11705
Biosample Accessionbs_r11705
Biomaterial Descriptionseedling, green parts
Biomaterial Is Control?False
Growth DescriptionSeeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C).
Treatment DescriptionDrought stress: The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels back in the climate chamber. Harvested 3 h after start of experiment
Anatomy Descriptionall green parts
Development Description16 days old plants, green parts
Probe Typetotal RNA
Germplasm NameCol-0
Germplasm TAIR Accession1092
Replicate Set Typebiological
Is Multichannel?False
Is Recerse Dye?False
Scanning SoftwareGCOS
Array DesignATH1-121501
Number Of Slides2


Replicate Set Accession: rs_r11709
Experiment Accession11700
Replicate Set Accessionrs_r11709
Biosample Accessionbs_r11709
Biomaterial Descriptionseedling, green parts
Biomaterial Is Control?False
Growth DescriptionSeeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C).
Treatment DescriptionDrought stress: The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels back in the climate chamber. Harvested 12 h after start of experiment
Anatomy Descriptionall green parts
Development Description16 days old plants, green parts
Probe Typetotal RNA
Germplasm NameCol-0
Germplasm TAIR Accession1092
Replicate Set Typebiological
Is Multichannel?False
Is Recerse Dye?False
Scanning SoftwareGCOS
Array DesignATH1-121501
Number Of Slides2


Replicate Set Accession: rs_r11707
Experiment Accession11700
Replicate Set Accessionrs_r11707
Biosample Accessionbs_r11707
Biomaterial Descriptionseedling, green parts
Biomaterial Is Control?False
Growth DescriptionSeeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C).
Treatment DescriptionDrought stress: The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels back in the climate chamber. Harvested 6 h after start of experiment
Anatomy Descriptionall green parts
Development Description16 days old plants, green parts
Probe Typetotal RNA
Germplasm NameCol-0
Germplasm TAIR Accession1092
Replicate Set Typebiological
Is Multichannel?False
Is Recerse Dye?False
Scanning SoftwareGCOS
Array DesignATH1-121501
Number Of Slides2


Replicate Set Accession: rs_r11703
Experiment Accession11700
Replicate Set Accessionrs_r11703
Biosample Accessionbs_r11703
Biomaterial Descriptionseedling, green parts
Biomaterial Is Control?False
Growth DescriptionSeeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C).
Treatment DescriptionDrought stress: The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels back in the climate chamber. Harvested 1 h after start of experiment
Anatomy Descriptionall green parts
Development Description16 days old plants, green parts
Probe Typetotal RNA
Germplasm NameCol-0
Germplasm TAIR Accession1092
Replicate Set Typebiological
Is Multichannel?False
Is Recerse Dye?False
Scanning SoftwareGCOS
Array DesignATH1-121501
Number Of Slides2


Replicate Set Accession: rs_r11713
Experiment Accession11700
Replicate Set Accessionrs_r11713
Biosample Accessionbs_r11713
Biomaterial Descriptionseedling, green parts
Biomaterial Is Control?False
Growth DescriptionSeeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C).
Treatment DescriptionDrought stress: The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels back in the climate chamber. Harvested 0,25 h after start of experiment
Anatomy Descriptionall green parts
Development Description16 days old plants, green parts
Probe Typetotal RNA
Germplasm NameCol-0
Germplasm TAIR Accession1092
Replicate Set Typebiological
Is Multichannel?False
Is Recerse Dye?False
Scanning SoftwareGCOS
Array DesignATH1-121501
Number Of Slides2


Replicate Set Accession: rs_r11702
Experiment Accession11700
Replicate Set Accessionrs_r11702
Biosample Accessionbs_r11702
Biomaterial Descriptionseedling, roots
Biomaterial Is Control?False
Growth DescriptionSeeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C).
Treatment DescriptionDrought stress: The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels back in the climate chamber. Harvested 0,5 h after start of experiment
Anatomy Descriptionall roots
Development Description16 days old plants, roots
Probe Typetotal RNA
Germplasm NameCol-0
Germplasm TAIR Accession1092
Replicate Set Typebiological
Is Multichannel?False
Is Recerse Dye?False
Scanning SoftwareGCOS
Array DesignATH1-121501
Number Of Slides2


Replicate Set Accession: rs_r11714
Experiment Accession11700
Replicate Set Accessionrs_r11714
Biosample Accessionbs_r11714
Biomaterial Descriptionseedling, roots
Biomaterial Is Control?False
Growth DescriptionSeeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C).
Treatment DescriptionDrought stress: The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels back in the climate chamber. Harvested 0,25 h after start of experiment
Anatomy Descriptionall roots
Development Description16 days old plants, roots
Probe Typetotal RNA
Germplasm NameCol-0
Germplasm TAIR Accession1092
Replicate Set Typebiological
Is Multichannel?False
Is Recerse Dye?False
Scanning SoftwareGCOS
Array DesignATH1-121501
Number Of Slides2


Replicate Set Accession: rs_r11712
Experiment Accession11700
Replicate Set Accessionrs_r11712
Biosample Accessionbs_r11712
Biomaterial Descriptionseedling, roots
Biomaterial Is Control?False
Growth DescriptionSeeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C).
Treatment DescriptionDrought stress: The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels back in the climate chamber. Harvested 24 h after start of experiment
Anatomy Descriptionall roots
Development Description16 days old plants, roots
Probe Typetotal RNA
Germplasm NameCol-0
Germplasm TAIR Accession1092
Replicate Set Typebiological
Is Multichannel?False
Is Recerse Dye?False
Scanning SoftwareGCOS
Array DesignATH1-121501
Number Of Slides2


Replicate Set Accession: rs_r11704
Experiment Accession11700
Replicate Set Accessionrs_r11704
Biosample Accessionbs_r11704
Biomaterial Descriptionseedling, roots
Biomaterial Is Control?False
Growth DescriptionSeeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C).
Treatment DescriptionDrought stress: The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels back in the climate chamber. Harvested 1 h after start of experiment
Anatomy Descriptionall roots
Development Description16 days old plants, roots
Probe Typetotal RNA
Germplasm NameCol-0
Germplasm TAIR Accession1092
Replicate Set Typebiological
Is Multichannel?False
Is Recerse Dye?False
Scanning SoftwareGCOS
Array DesignATH1-121501
Number Of Slides2


Replicate Set Accession: rs_r11701
Experiment Accession11700
Replicate Set Accessionrs_r11701
Biosample Accessionbs_r11701
Biomaterial Descriptionseedling, green parts
Biomaterial Is Control?False
Growth DescriptionSeeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C).
Treatment DescriptionDrought stress: The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels back in the climate chamber. Harvested 0,5 h after start of experiment
Anatomy Descriptionall green parts
Development Description16 days old plants, green parts
Probe Typetotal RNA
Germplasm NameCol-0
Germplasm TAIR Accession1092
Replicate Set Typebiological
Is Multichannel?False
Is Recerse Dye?False
Scanning SoftwareGCOS
Array DesignATH1-121501
Number Of Slides2


Replicate Set Accession: rs_r11708
Experiment Accession11700
Replicate Set Accessionrs_r11708
Biosample Accessionbs_r11708
Biomaterial Descriptionseedling, roots
Biomaterial Is Control?False
Growth DescriptionSeeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C).
Treatment DescriptionDrought stress: The plants were stressed by 15 min. dry air stream (clean bench) until 10% loss of fresh weight; then incubation in closed vessels back in the climate chamber. Harvested 6 h after start of experiment
Anatomy Descriptionall roots
Development Description16 days old plants, roots
Probe Typetotal RNA
Germplasm NameCol-0
Germplasm TAIR Accession1092
Replicate Set Typebiological
Is Multichannel?False
Is Recerse Dye?False
Scanning SoftwareGCOS
Array DesignATH1-121501
Number Of Slides2