|
Replicate Set Accession: rs_r12111 |
Experiment Accession | 12100 |
Replicate Set Accession | rs_r12111 |
Biosample Accession | bs_r12111 |
Biomaterial Description | seedling, green parts |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | UV-B stress: The plants were stressed by 0,25 h UV-B light field consisting of six Philips TL 40W/12 UV fluorescent tubes (?max. 310 nm, half-bandwidth 40 nm, fluence rate 7 W/m2) filtered through 3 mm transmission cutoff filters of the WG series ((WG295, WG305, and WG327; Schott,Mainz, Germany). Harvested 24 h after start of experiment. |
Anatomy Description | all green parts |
Development Description | 16 days old plants, green parts |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12103 |
Experiment Accession | 12100 |
Replicate Set Accession | rs_r12103 |
Biosample Accession | bs_r12103 |
Biomaterial Description | seedling, green parts |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | UV-B stress: The plants were stressed by 0,25 h UV-B light field consisting of six Philips TL 40W/12 UV fluorescent tubes (?max. 310 nm, half-bandwidth 40 nm, fluence rate 7 W/m2) filtered through 3 mm transmission cutoff filters of the WG series ((WG295, WG305, and WG327; Schott,Mainz, Germany). Harvested 1 h after start of experiment. |
Anatomy Description | all green parts |
Development Description | 16 days old plants, green parts |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12105 |
Experiment Accession | 12100 |
Replicate Set Accession | rs_r12105 |
Biosample Accession | bs_r12105 |
Biomaterial Description | seedling, green parts |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | UV-B stress: The plants were stressed by 0,25 h UV-B light field consisting of six Philips TL 40W/12 UV fluorescent tubes (?max. 310 nm, half-bandwidth 40 nm, fluence rate 7 W/m2) filtered through 3 mm transmission cutoff filters of the WG series ((WG295, WG305, and WG327; Schott,Mainz, Germany). Harvested 3 h after start of experiment. |
Anatomy Description | all green parts |
Development Description | 16 days old plants, green parts |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12110 |
Experiment Accession | 12100 |
Replicate Set Accession | rs_r12110 |
Biosample Accession | bs_r12110 |
Biomaterial Description | seedling, roots |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | UV-B stress: The plants were stressed by 0,25 h UV-B light field consisting of six Philips TL 40W/12 UV fluorescent tubes (?max. 310 nm, half-bandwidth 40 nm, fluence rate 7 W/m2) filtered through 3 mm transmission cutoff filters of the WG series ((WG295, WG305, and WG327; Schott,Mainz, Germany). Harvested 12 h after start of experiment. |
Anatomy Description | all roots |
Development Description | 16 days old plants, roots |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12102 |
Experiment Accession | 12100 |
Replicate Set Accession | rs_r12102 |
Biosample Accession | bs_r12102 |
Biomaterial Description | seedling, roots |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | UV-B stress: The plants were stressed by 0,25 h UV-B light field consisting of six Philips TL 40W/12 UV fluorescent tubes (?max. 310 nm, half-bandwidth 40 nm, fluence rate 7 W/m2) filtered through 3 mm transmission cutoff filters of the WG series ((WG295, WG305, and WG327; Schott,Mainz, Germany). Harvested 0,5 h after start of experiment. |
Anatomy Description | all roots |
Development Description | 16 days old plants, roots |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12106 |
Experiment Accession | 12100 |
Replicate Set Accession | rs_r12106 |
Biosample Accession | bs_r12106 |
Biomaterial Description | seedling, roots |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | UV-B stress: The plants were stressed by 0,25 h UV-B light field consisting of six Philips TL 40W/12 UV fluorescent tubes (?max. 310 nm, half-bandwidth 40 nm, fluence rate 7 W/m2) filtered through 3 mm transmission cutoff filters of the WG series ((WG295, WG305, and WG327; Schott,Mainz, Germany). Harvested 3 h after start of experiment. |
Anatomy Description | all roots |
Development Description | 16 days old plants, roots |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12107 |
Experiment Accession | 12100 |
Replicate Set Accession | rs_r12107 |
Biosample Accession | bs_r12107 |
Biomaterial Description | seedling, green parts |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | UV-B stress: The plants were stressed by 0,25 h UV-B light field consisting of six Philips TL 40W/12 UV fluorescent tubes (?max. 310 nm, half-bandwidth 40 nm, fluence rate 7 W/m2) filtered through 3 mm transmission cutoff filters of the WG series ((WG295, WG305, and WG327; Schott,Mainz, Germany). Harvested 6 h after start of experiment. |
Anatomy Description | all green parts |
Development Description | 16 days old plants, green parts |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12109 |
Experiment Accession | 12100 |
Replicate Set Accession | rs_r12109 |
Biosample Accession | bs_r12109 |
Biomaterial Description | seedling, green parts |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | UV-B stress: The plants were stressed by 0,25 h UV-B light field consisting of six Philips TL 40W/12 UV fluorescent tubes (?max. 310 nm, half-bandwidth 40 nm, fluence rate 7 W/m2) filtered through 3 mm transmission cutoff filters of the WG series ((WG295, WG305, and WG327; Schott,Mainz, Germany). Harvested 12 h after start of experiment. |
Anatomy Description | all green parts |
Development Description | 16 days old plants, green parts |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12114 |
Experiment Accession | 12100 |
Replicate Set Accession | rs_r12114 |
Biosample Accession | bs_r12114 |
Biomaterial Description | seedling, roots |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | UV-B stress: The plants were stressed by 0,25 h UV-B light field consisting of six Philips TL 40W/12 UV fluorescent tubes (?max. 310 nm, half-bandwidth 40 nm, fluence rate 7 W/m2) filtered through 3 mm transmission cutoff filters of the WG series ((WG295, WG305, and WG327; Schott,Mainz, Germany). Harvested 0,25 h after start of experiment. |
Anatomy Description | all roots |
Development Description | 16 days old plants, roots |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12108 |
Experiment Accession | 12100 |
Replicate Set Accession | rs_r12108 |
Biosample Accession | bs_r12108 |
Biomaterial Description | seedling, roots |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | UV-B stress: The plants were stressed by 0,25 h UV-B light field consisting of six Philips TL 40W/12 UV fluorescent tubes (?max. 310 nm, half-bandwidth 40 nm, fluence rate 7 W/m2) filtered through 3 mm transmission cutoff filters of the WG series ((WG295, WG305, and WG327; Schott,Mainz, Germany). Harvested 6 h after start of experiment. |
Anatomy Description | all roots |
Development Description | 16 days old plants, roots |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12113 |
Experiment Accession | 12100 |
Replicate Set Accession | rs_r12113 |
Biosample Accession | bs_r12113 |
Biomaterial Description | seedling, green parts |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | UV-B stress: The plants were stressed by 0,25 h UV-B light field consisting of six Philips TL 40W/12 UV fluorescent tubes (?max. 310 nm, half-bandwidth 40 nm, fluence rate 7 W/m2) filtered through 3 mm transmission cutoff filters of the WG series ((WG295, WG305, and WG327; Schott,Mainz, Germany). Harvested 0,25 h after start of experiment. |
Anatomy Description | all green parts |
Development Description | 16 days old plants, green parts |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12101 |
Experiment Accession | 12100 |
Replicate Set Accession | rs_r12101 |
Biosample Accession | bs_r12101 |
Biomaterial Description | seedling, green parts |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | UV-B stress: The plants were stressed by 0,25 h UV-B light field consisting of six Philips TL 40W/12 UV fluorescent tubes (?max. 310 nm, half-bandwidth 40 nm, fluence rate 7 W/m2) filtered through 3 mm transmission cutoff filters of the WG series ((WG295, WG305, and WG327; Schott,Mainz, Germany). Harvested 0,5 h after start of experiment. |
Anatomy Description | all green parts |
Development Description | 16 days old plants, green parts |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12104 |
Experiment Accession | 12100 |
Replicate Set Accession | rs_r12104 |
Biosample Accession | bs_r12104 |
Biomaterial Description | seedling, roots |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | UV-B stress: The plants were stressed by 0,25 h UV-B light field consisting of six Philips TL 40W/12 UV fluorescent tubes (?max. 310 nm, half-bandwidth 40 nm, fluence rate 7 W/m2) filtered through 3 mm transmission cutoff filters of the WG series ((WG295, WG305, and WG327; Schott,Mainz, Germany). Harvested 1 h after start of experiment. |
Anatomy Description | all roots |
Development Description | 16 days old plants, roots |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12112 |
Experiment Accession | 12100 |
Replicate Set Accession | rs_r12112 |
Biosample Accession | bs_r12112 |
Biomaterial Description | seedling, roots |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | UV-B stress: The plants were stressed by 0,25 h UV-B light field consisting of six Philips TL 40W/12 UV fluorescent tubes (?max. 310 nm, half-bandwidth 40 nm, fluence rate 7 W/m2) filtered through 3 mm transmission cutoff filters of the WG series ((WG295, WG305, and WG327; Schott,Mainz, Germany). Harvested 24 h after start of experiment. |
Anatomy Description | all roots |
Development Description | 16 days old plants, roots |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
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