|
Replicate Set Accession: rs_r12202 |
Experiment Accession | 12200 |
Replicate Set Accession | rs_r12202 |
Biosample Accession | bs_r12202 |
Biomaterial Description | seedling, roots |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | Wound stress: The plants were wounded by punctuation of the leaves with a custom made pin-tool consisting of 16 needles (~2 needles/1 cm2). Three times of consecutive application pierced on average three to four distinct wholes per leaf. Harvested 0,5 h after start of experiment |
Anatomy Description | all roots |
Development Description | 16 days old plants, roots |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12212 |
Experiment Accession | 12200 |
Replicate Set Accession | rs_r12212 |
Biosample Accession | bs_r12212 |
Biomaterial Description | seedling, roots |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | Wound stress: The plants were wounded by punctuation of the leaves with a custom made pin-tool consisting of 16 needles (~2 needles/1 cm2). Three times of consecutive application pierced on average three to four distinct wholes per leaf. Harvested 24 h after start of experiment |
Anatomy Description | all roots |
Development Description | 16 days old plants, roots |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12206 |
Experiment Accession | 12200 |
Replicate Set Accession | rs_r12206 |
Biosample Accession | bs_r12206 |
Biomaterial Description | seedling, roots |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | Wound stress: The plants were wounded by punctuation of the leaves with a custom made pin-tool consisting of 16 needles (~2 needles/1 cm2). Three times of consecutive application pierced on average three to four distinct wholes per leaf. Harvested 3 h after start of experiment |
Anatomy Description | all roots |
Development Description | 16 days old plants, roots |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12201 |
Experiment Accession | 12200 |
Replicate Set Accession | rs_r12201 |
Biosample Accession | bs_r12201 |
Biomaterial Description | seedling, green parts |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | Wound stress: The plants were wounded by punctuation of the leaves with a custom made pin-tool consisting of 16 needles (~2 needles/1 cm2). Three times of consecutive application pierced on average three to four distinct wholes per leaf. Harvested 0,5 h after start of experiment |
Anatomy Description | all green parts |
Development Description | 16 days old plants, green parts |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12213 |
Experiment Accession | 12200 |
Replicate Set Accession | rs_r12213 |
Biosample Accession | bs_r12213 |
Biomaterial Description | seedling, green parts |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | Wound stress: The plants were wounded by punctuation of the leaves with a custom made pin-tool consisting of 16 needles (~2 needles/1 cm2). Three times of consecutive application pierced on average three to four distinct wholes per leaf. Harvested 0,25 h after start of experiment |
Anatomy Description | all green parts |
Development Description | 16 days old plants, green parts |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12211 |
Experiment Accession | 12200 |
Replicate Set Accession | rs_r12211 |
Biosample Accession | bs_r12211 |
Biomaterial Description | seedling, green parts |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | Wound stress: The plants were wounded by punctuation of the leaves with a custom made pin-tool consisting of 16 needles (~2 needles/1 cm2). Three times of consecutive application pierced on average three to four distinct wholes per leaf. Harvested 24 h after start of experiment |
Anatomy Description | all green parts |
Development Description | 16 days old plants, green parts |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12209 |
Experiment Accession | 12200 |
Replicate Set Accession | rs_r12209 |
Biosample Accession | bs_r12209 |
Biomaterial Description | seedling, green parts |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | Wound stress: The plants were wounded by punctuation of the leaves with a custom made pin-tool consisting of 16 needles (~2 needles/1 cm2). Three times of consecutive application pierced on average three to four distinct wholes per leaf. Harvested 12 h after start of experiment |
Anatomy Description | all green parts |
Development Description | 16 days old plants, green parts |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12210 |
Experiment Accession | 12200 |
Replicate Set Accession | rs_r12210 |
Biosample Accession | bs_r12210 |
Biomaterial Description | seedling, roots |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | Wound stress: The plants were wounded by punctuation of the leaves with a custom made pin-tool consisting of 16 needles (~2 needles/1 cm2). Three times of consecutive application pierced on average three to four distinct wholes per leaf. Harvested 12 h after start of experiment |
Anatomy Description | all roots |
Development Description | 16 days old plants, roots |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12208 |
Experiment Accession | 12200 |
Replicate Set Accession | rs_r12208 |
Biosample Accession | bs_r12208 |
Biomaterial Description | seedling, roots |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | Wound stress: The plants were wounded by punctuation of the leaves with a custom made pin-tool consisting of 16 needles (~2 needles/1 cm2). Three times of consecutive application pierced on average three to four distinct wholes per leaf. Harvested 6 h after start of experiment |
Anatomy Description | all roots |
Development Description | 16 days old plants, roots |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12205 |
Experiment Accession | 12200 |
Replicate Set Accession | rs_r12205 |
Biosample Accession | bs_r12205 |
Biomaterial Description | seedling, green parts |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | Wound stress: The plants were wounded by punctuation of the leaves with a custom made pin-tool consisting of 16 needles (~2 needles/1 cm2). Three times of consecutive application pierced on average three to four distinct wholes per leaf. Harvested 3 h after start of experiment |
Anatomy Description | all green parts |
Development Description | 16 days old plants, green parts |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12204 |
Experiment Accession | 12200 |
Replicate Set Accession | rs_r12204 |
Biosample Accession | bs_r12204 |
Biomaterial Description | seedling, roots |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | Wound stress: The plants were wounded by punctuation of the leaves with a custom made pin-tool consisting of 16 needles (~2 needles/1 cm2). Three times of consecutive application pierced on average three to four distinct wholes per leaf. Harvested 1 h after start of experiment |
Anatomy Description | all roots |
Development Description | 16 days old plants, roots |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12203 |
Experiment Accession | 12200 |
Replicate Set Accession | rs_r12203 |
Biosample Accession | bs_r12203 |
Biomaterial Description | seedling, green parts |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | Wound stress: The plants were wounded by punctuation of the leaves with a custom made pin-tool consisting of 16 needles (~2 needles/1 cm2). Three times of consecutive application pierced on average three to four distinct wholes per leaf. Harvested 1 h after start of experiment |
Anatomy Description | all green parts |
Development Description | 16 days old plants, green parts |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12214 |
Experiment Accession | 12200 |
Replicate Set Accession | rs_r12214 |
Biosample Accession | bs_r12214 |
Biomaterial Description | seedling, roots |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | Wound stress: The plants were wounded by punctuation of the leaves with a custom made pin-tool consisting of 16 needles (~2 needles/1 cm2). Three times of consecutive application pierced on average three to four distinct wholes per leaf. Harvested 0,25 h after start of experiment |
Anatomy Description | all roots |
Development Description | 16 days old plants, roots |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12207 |
Experiment Accession | 12200 |
Replicate Set Accession | rs_r12207 |
Biosample Accession | bs_r12207 |
Biomaterial Description | seedling, green parts |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | Wound stress: The plants were wounded by punctuation of the leaves with a custom made pin-tool consisting of 16 needles (~2 needles/1 cm2). Three times of consecutive application pierced on average three to four distinct wholes per leaf. Harvested 6 h after start of experiment |
Anatomy Description | all green parts |
Development Description | 16 days old plants, green parts |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
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