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NOTIFICATION: Migration to a new permanent home at wwwcsbdb.de.
Herewith we inform you that all CSB.DB databases have been migrated at the beginning of 2016. This includes all gene correlation and expression databases (search the WWW for alternatives), the GMD@CSB.DB module (alternative will be reachable via http://gmd.mpimp-golm.mpg.de/) and all associated databases (terminated).
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11900
 

Experiment Accession: 11900
Experiment Accession11900
Project nameAtGenExpress
OrganismArabidopsis thaliana
Data SourceRZPD
Experiment Category chemical treatment, tissue comparison
Experiment Goals response to oxidative stress
Experimental Variables oxidative stress, tissue, time course
Is series?time course
environment is variable?True
sample material is variable?False
probe type is variable?False
harvesting is variable?True
Experiment Namebasic chemical treatment
Experiment DescriptionVarious environmental stresses like high salinity, scarcity of water or heat result in oxidative stress as they stimulate the accumulation of reactive oxygen species (ROS). Oxidative stress is thought to be one of the major causes of cellular damage as a result of stress (Bartels, 2001; Mittler, 2002; Ramanjulu and Bartels, 2002). The amplification of ROS damage is further stimulated by the accumulation of additional damaging molecules and degradation products, e.g. toxic aldehydes, arising from the reactions of ROS with membrane lipids. The detoxification process is accompanied by differential gene expression. We are especially interested in the expression profile of members of the aldehyde dehydrogenase gene superfamily which are thought to take part in the detoxification process caused by oxidative stress (Kirch et al., 2001; Sunkar et al., 2003). Methyl viologen was chosen as the stress inducing chemical because it can bind to thylakoid membranes of the chloroplast and transfer the electrons to O2 in a chain reaction which causes continuous formation of superoxide radicals in the presence of light (Asada, 1996). Additionally, the substance is known to be stable in an aqueous solution for a longer period of time so that it is sufficient to add it only once at the beginning of the experiment. Asada K. (1996) Radical production and scavenging in the chloroplasts. In Photosynthesis and the Environment (Baker, N.R., ed.). Dordrecht, the Netherlands: Kluwer Academic, pp. 123-150. Bartels D. (2001) Trends Plant Sci. 6, 284-286. Kirch et al. (2001) Plant J. 28, 555-567. Mittler R. (2002) Trends Plant Sci. 7, 405-410. Ramanjulu and Bartels (2002) Plant Cell Environ. 25, 141-151. Sunkar et al. (2003) Plant J. 35, 452-464.
Number of Replicate Sets12
Number of BioSamples12
Number of Slides24
Commentscontrol experiment: 11300