The Comprehensive Systems Biology Project (CSB)
csbdb GMD@CSB.DB
- The Golm Metabolome Database -
Hosted at Max Planck Institute of Molecular Plant Physiology
Databases: Associated DB | Transcriptome DB | Metabolome DB | Co-Response DB | BestFit
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NOTIFICATION: GMD has been migrated and will permanently be maintained on private servers.
Herewith we inform you that all CSB.DB databases have been migrated and associated services will be maintained on private servers since the beginning of 2016. This includes all gene correlation and expression databases (search the WWW for alternatives), the GMD@CSB.DB module (alternative will be reachable via and all associated databases (terminated).
The BestFit software, a tool for non-aqueous fractionation data analysis, will be available by the Experimental Systems Biology Research Group headed by Dr. Patrick Giavalisco.
GMD@CSB.DB: The Analytic Technology Pages
The Analytic@GMD Pages harbours information related to analytical technologies, methods and protocols integrated in GMD as well as and other necessary information related to the field of metabolomics. For in-depth information as well as collaborations we recommended contacting the responsible scientist(s) by the given mail contacts.

To get information of your choice use the links listed below.

Method Derivatization Extraction Through-put (max.) Citation Group
GC-MS Metabolite profiling M[1] GC-TOF-MS (EITTMS) MEOX TMS hot methanol / chloroform 150-190/ week Wagner, C., Sefkow, M., Kopka, J. (2003). Phytochemistry, 62, 887-900. Kopka,J.
M[2] GC-QUAD-MS (EIQTMS) MEOX TMS 80-100/ week  
M[4] GC-IONTRAP-MS TBS hot Bieleski solvent 10-20/ week Birkemeyer, C., Kolasa, A., Kopka, J. (2003). Journal of Chromatography A, 993, 89-102.
HPLC C18-nucleosyl none ethylacetate fraction n.d.   Gibon,Y.
C18-nucleosyl none methanol fraction n.d.  
C18-reverse phase OPA ethanol n.d.  
Ion exchange none TCA n.d.  
reversed phase Dansyl   n.d.   Hincha,D.
HPAEC-PAD Ion exchange none ethanol n.d.  
MTP (Colorimetric assay) Ninhydrine   ethanol 186/ day   Gibon,Y.
Bradford none ethanol 500-1000/ week  
MTP (Enzyme assay) Endpoint   ethanol 186/ day  
Endpoint + Cycling   ethanol 186/ day Gibon, Y., Vigeolas, H., Tiessen, A., Geigenberger, P., Stitt, M. (2002). Plant Journal, 30, 221-235
Cycling   TCA <100/ day Gibon, Y., Vigeolas, H., Tiessen, A., Geigenberger, P., Stitt, M. (2002). Plant Journal, 30, 221-235
Endpoint   NaOH 500-1000/ week  
MTP (Fluorimetric assay)     ethanol 186/ day  
MTP (Spectrophotometric assay)     ethanol 186/ day  

For suggestions and questions feel free to contact the CSB.DB curator.
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