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Arabidopsis thaliana - Expression Data Matrix nasc0271 (m0271): Processing

Transcript profiles of Matrix nasc0271 (m0271) were derived from the NASC's Affymetrix Facility (NASCArrays, Craigon et al., 2004) and originated from 22k oligonucleotide microarray technology (Affymetrix). The transcript profiling experiments of nasc0271 (m0271) covered miscellaneous treatments and characterisation of mutants. In order to reduce the number of missing data nasc0271 (m0271) was designed according to the following criteria. Each particular gene was well measured in at least 85% of the experiments (Detection Call: 'M' or 'P'). 15% of measurements were tolerated to be absent (Detection Call: 'A'). Negative transcript intensities were treated as missing value and genes with more than 5% missing values were ignored. To avoid overrepresentation of experimental conditions the chosen number of replicates per experimental condition was approximately equal. Matrix nasc0271 (m0271) comprised 51 experiments, which were described by 9694 genes. Normalised transcript intensities were log base 2 transformed. Co-Response analyses were performed on range normalised (genes) matrix.