|
Replicate Set Accession: rs_r12006 |
Experiment Accession | 12000 |
Replicate Set Accession | rs_r12006 |
Biosample Accession | bs_r12006 |
Biomaterial Description | seedling, roots |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | 1.0h of 38°C heat stress in an incubator |
Anatomy Description | all roots |
Development Description | 16 days old plants, roots |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12015 |
Experiment Accession | 12000 |
Replicate Set Accession | rs_r12015 |
Biosample Accession | bs_r12015 |
Biomaterial Description | seedling, green parts |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | 3.0h of 38°C heat stress in an incubator followed by 21.0h recovery at 25°C |
Anatomy Description | all green parts |
Development Description | 16 days old plants, green parts |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12003 |
Experiment Accession | 12000 |
Replicate Set Accession | rs_r12003 |
Biosample Accession | bs_r12003 |
Biomaterial Description | seedling, green parts |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | 0.5h of 38°C heat stress in an incubator |
Anatomy Description | all green parts |
Development Description | 16 days old plants, green parts |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12013 |
Experiment Accession | 12000 |
Replicate Set Accession | rs_r12013 |
Biosample Accession | bs_r12013 |
Biomaterial Description | seedling, green parts |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | 3.0h of 38°C heat stress in an incubator followed by 9.0h recovery at 25°C |
Anatomy Description | all green parts |
Development Description | 16 days old plants, green parts |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12008 |
Experiment Accession | 12000 |
Replicate Set Accession | rs_r12008 |
Biosample Accession | bs_r12008 |
Biomaterial Description | seedling, roots |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | 3.0h of 38°C heat stress in an incubator |
Anatomy Description | all roots |
Development Description | 16 days old plants, roots |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12012 |
Experiment Accession | 12000 |
Replicate Set Accession | rs_r12012 |
Biosample Accession | bs_r12012 |
Biomaterial Description | seedling, roots |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | 3.0h of 38°C heat stress in an incubator followed by 3.0h recovery at 25°C |
Anatomy Description | all roots |
Development Description | 16 days old plants, roots |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12010 |
Experiment Accession | 12000 |
Replicate Set Accession | rs_r12010 |
Biosample Accession | bs_r12010 |
Biomaterial Description | seedling, roots |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | 3.0h of 38°C heat stress in an incubator followed by 1.0h recovery at 25°C |
Anatomy Description | all roots |
Development Description | 16 days old plants, roots |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12007 |
Experiment Accession | 12000 |
Replicate Set Accession | rs_r12007 |
Biosample Accession | bs_r12007 |
Biomaterial Description | seedling, green parts |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | 3.0h of 38°C heat stress in an incubator |
Anatomy Description | all green parts |
Development Description | 16 days old plants, green parts |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12005 |
Experiment Accession | 12000 |
Replicate Set Accession | rs_r12005 |
Biosample Accession | bs_r12005 |
Biomaterial Description | seedling, green parts |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | 1.0h of 38°C heat stress in an incubator |
Anatomy Description | all green parts |
Development Description | 16 days old plants, green parts |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12011 |
Experiment Accession | 12000 |
Replicate Set Accession | rs_r12011 |
Biosample Accession | bs_r12011 |
Biomaterial Description | seedling, green parts |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | 3.0h of 38°C heat stress in an incubator followed by 3.0h recovery at 25°C |
Anatomy Description | all green parts |
Development Description | 16 days old plants, green parts |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12004 |
Experiment Accession | 12000 |
Replicate Set Accession | rs_r12004 |
Biosample Accession | bs_r12004 |
Biomaterial Description | seedling, roots |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | 0.5h of 38°C heat stress in an incubator |
Anatomy Description | all roots |
Development Description | 16 days old plants, roots |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12014 |
Experiment Accession | 12000 |
Replicate Set Accession | rs_r12014 |
Biosample Accession | bs_r12014 |
Biomaterial Description | seedling, roots |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | 3.0h of 38°C heat stress in an incubator followed by 9.0h recovery at 25°C |
Anatomy Description | all roots |
Development Description | 16 days old plants, roots |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12001 |
Experiment Accession | 12000 |
Replicate Set Accession | rs_r12001 |
Biosample Accession | bs_r12001 |
Biomaterial Description | seedling, green parts |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | 0.25h of 38°C heat stress in an incubator |
Anatomy Description | all green parts |
Development Description | 16 days old plants, green parts |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12016 |
Experiment Accession | 12000 |
Replicate Set Accession | rs_r12016 |
Biosample Accession | bs_r12016 |
Biomaterial Description | seedling, roots |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | 3.0h of 38°C heat stress in an incubator followed by 21.0h recovery at 25°C |
Anatomy Description | all roots |
Development Description | 16 days old plants, roots |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12009 |
Experiment Accession | 12000 |
Replicate Set Accession | rs_r12009 |
Biosample Accession | bs_r12009 |
Biomaterial Description | seedling, green parts |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | 3.0h of 38°C heat stress in an incubator followed by 1.0h recovery at 25°C |
Anatomy Description | all green parts |
Development Description | 16 days old plants, green parts |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
Replicate Set Accession: rs_r12002 |
Experiment Accession | 12000 |
Replicate Set Accession | rs_r12002 |
Biosample Accession | bs_r12002 |
Biomaterial Description | seedling, roots |
Biomaterial Is Control? | False |
Growth Description | Seeds of Arabidopsis thaliana Wilde Type (col-0) were sown on rafts in Magenta boxes containing MS-Agar-media (MS-Agar-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); 0,5% sucrose (5g for 1L); Adjust pH to 5,7 with KOH; Add 5 g of plant Agar (Ducheva); Autoclave for 12 min at 121°C; Poor 50 ml of media in each sterile Magenta box with ventilation). After 2 days in the cold room (4°C, dark) the boxes were transferred to the long day chamber. Long day conditions were 16/8 hrs light/dark, 24°C, 50% humidity and 150 µEinstein/cm2 sec light intensity. At day 11 the rafts were transferred in Magenta boxes containing MS-liquid-media (MS-liquid-media (1L): 0,5x MS media + Gamborg B5 vitamins (Ducheva)(2,2g for 1L); Adjust pH to 5,7 with KOH; Poor 75 ml of media in each Magenta box without ventilation containing a floater ; Autoclave for 12 min at 121°C). |
Treatment Description | 0.25h of 38°C heat stress in an incubator |
Anatomy Description | all roots |
Development Description | 16 days old plants, roots |
Probe Type | total RNA |
Germplasm Name | Col-0 |
Germplasm TAIR Accession | 1092 |
Replicate Set Type | biological |
Is Multichannel? | False |
Is Recerse Dye? | False |
Scanning Software | GCOS |
Array Design | ATH1-121501 |
Number Of Slides | 2 |
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